Resources
- FAQ
- Protocol
- Cell Culture Guide
- Technical Bulletins
-
Explore & Learn
-
Cell Biology
- How to Handle Mycoplasma in Cell Culture?
- Enrichment, Isolation and Characterization of Circulating Tumor Cells (CTCs)
- Strategies for Enrichment of Circulating Tumor Cells (CTCs)
- How to Assess the Migratory and Invasive Capacity of Cells?
- Multi-Differentiation of Peripheral Blood Mononuclear Cells
- Organoid Differentiation from Induced Pluripotent Stem Cells
- Tips For Cell Cryopreservation
- What Cell Lines Are Commonly Used in Biopharmaceutical Production?
- Troubleshooting Cell Culture Contamination: A Comprehensive Guide
- How to Isolate and Analyze Tumor-Infiltrating Leukocytes?
- Contamination of Cell Cultures & Treatment
- Generation and Applications of Neural Stem Cells
- Stem Cell Markers
- Comparison of the MSCs from Different Sources
- T Cell Activation and Expansion
- Quantification of Cytokines
- Mesenchymal Stem Cells: A Comprehensive Exploration
- What are the Differences Between M1 and M2 Macrophages?
- How to Decide Between 2D and 3D Cell Cultures?
- Neural Differentiation from Induced Pluripotent Stem Cells
- Biomarkers and Signaling Pathways in Tumor Stem Cells
- Techniques for Cell Separation
- Circulating Tumor Cells as Cancer Biomarkers in the Clinic
- CFU Assay for Hematopoietic Cell
- Monocytes vs. Macrophages
- How to Detect and Remove Endotoxins in Biologics?
- Comparison of Different Methods to Measure Cell Viability
- How to Isolate PBMCs from Whole Blood?
- CHO Cell Line Development
- What Are Myeloid Cell Markers?
- Cryopreservation of Cells Step by Step
- What are PBMCs?
- How to Start Your Culture: Thawing Frozen Cells
- Guidelines for Cell Banking to Ensure the Safety of Biologics
- Unveiling the Molecular Secrets of Adipogenesis in MSCs
- Tumor Stem Cells: Identification, Isolation and Therapeutic Interventions
- Isolation, Expansion, and Analysis of Natural Killer Cells
- Critical Quality Attributes and Assays for Induced Pluripotent Stem Cells
- Major Problems Caused by the Use of Uncharacterized Cell Lines
- T Cell, NK Cell Differentiation from Induced Pluripotent Stem Cells
- IL-12 Family Cytokines and Their Immune Functions
- Direct vs. Indirect Cell-Based ELISA
- Cell Culture Medium
- What Is Cell Proliferation and How to Analyze It?
- What are Mesothelial Cells?
- How to Scale Up Single-Cell Clones?
- STR Profiling—The ID Card of Cell Line
- Comparison of Several Techniques for the Detection of Apoptotic Cells
- Cell Cryopreservation Techniques and Practices
- How to Eliminate Mycoplasma From Cell Cultures?
- Human Primary Cells: Definition, Assay, Applications
- Understanding Immunogenicity Assays: A Comprehensive Guide
- Role of Cell-Based Assays in Drug Discovery and Development
- Mastering Cell Culture and Cryopreservation: Key Strategies for Optimal Cell Viability and Stability
- Adherent and Suspension Cell Culture
- Spatial Metabolomics Sloution
- Organoid Drug Screening
- Key Techniques in Primary, Immortalized and Stable Cell Line Development
- Histological Staining Techniques: From Traditional Chemical Staining to Immunohistochemistry
- From Primary to Immortalized: Navigating Key Cell Lines in Biomedical Research
- Overview of Cell Apoptosis Assays
- From Specimen to Slide: Core Methods in Histological Practice
- Modern Histological Techniques
- Exploring Cell Dynamics: Migration, Invasion, Adhesion, Angiogenesis, and EMT Assays
- Cell Viability, Proliferation and Cytotoxicity Assays
-
Histology
- Troubleshooting in Fluorescent Staining
- Multiple Animal Tissue Arrays
- Tips for Choosing the Right Protease Inhibitor
- Instructions for Tumour Tissue Collection, Storage and Dissociation
- Fluorescent Nuclear Staining Dyes
- Guides for Live Cell Imaging Dyes
- Overview of the FFPE Cell Pellet Product Lines
- Mitochondrial Staining
- Stains Used in Histology
- Immunohistochemistry Controls
- Comparison of Membrane Stains vs. Cell Surface Stains
- Microscope Platforms
- Overview of Common Tracking Labels for MSCs
- How to Apply NGS Technologies to FFPE Tissues?
- Cell Lysates: Composition, Properties, and Preparation
- Immunohistochemistry Troubleshooting
- Cell and Tissue Fixation
-
Exosome
- What's the Potential of PELN in Disease Treatment?
- Exosomes as Emerging Biomarker Tools for Diseases
- How to Apply Exosomes in Clinical?
- How to Efficiently Utilize MSC Exosomes for Disease Treatment?
- Summary of Approaches for Loading Cargo into Exosomes
- Emerging Technologies and Methodologies for Exosome Research
- How to Perform Targeted Modification of Exosomes?
- How to Enhancement Exosome Production?
- How to Label Exosomes?
- How to characterize exosomes?
- Classification, Isolation Techniques and Characterization of Exosomes
- Exosome Quality Control: How to Do It?
- Common Techniques for Exosome Nucleic Acid Extraction
- Exosome Transfection for Altering Biomolecular Delivery
- How do PELN Deliver Drugs?
- Current Research Status of Milk Exosomes
- Collection of Exosome Samples and Precautions
- Techniques for Exosome Quantification
- Exosome Antibodies
- How Important are Lipids in Exosome Composition and Biogenesis?
- Unraveling Biogenesis and Composition of Exosomes
- Production of Exosomes: Human Cell Lines and Cultivation Modes
- Applications of MSC-EVs in Immune Regulation and Regeneration
- What are the Functions of Exosomal Proteins?
- Exosome Size Measurement
- The Role of Exosomes in Cancer
-
ISH/FISH
- ISH probe labeling method
- Reagents Used in FISH Experiments
- What Types of Multicolor FISH Probe Sets Are Available?
- What Is the Use of FISH in Solid Tumors?
- Mapping of Transgenes by FISH
- Telomere Length Measurement Methods
- Comprehensive Comparison of IHC, CISH, and FISH Techniques
- FISH Tips and Troubleshooting
- Overview of Oligo-FISH Technology
- Differences Between DNA and RNA Probes
- Comparative Genomic Hybridization and Its Applications
- RNAscope ISH Technology
- CARD-FISH: Illuminating Microbial Diversity
- What are the Differences between FISH, aCGH, and NGS?
- Multiple Options for Proving Monoclonality
- FISH Techniques for Biofilm Detection
- Whole Chromosome Painting Probes for FISH
- In Situ Hybridization Probes
- Overview of Common FISH Techniques
- Multiple Approaches to Karyotyping
- What are Single, Dual, and Multiplex ISH?
- How to Use FISH in Hematologic Neoplasms?
- Different Types of FISH Probes for Oncology Research
- Small RNA Detection by ISH Methods
- Guidelines for the Design of FISH Probes
-
Toxicokinetics & Pharmacokinetics
- What Are Metabolism-Mediated Drug-Drug Interactions?
- How to Improve Drug Plasma Stability?
- Experimental Methods for Identifying Drug-Drug Interactions
- How to Conduct a Bioavailability Assessment?
- Organ-on-a-Chip Systems for Drug Screening
- How Is the Cytotoxicity of Drugs Determined?
- How to Improve the Pharmacokinetic Properties of Peptides?
- Toxicokinetics vs. Pharmacokinetics
- Pharmacokinetics Considerations for Antibody Drug Conjugates
- Key Considerations in Toxicokinetic
- Organoids in Drug Discovery: Revolutionizing Therapeutic Research
- Comparison of MDCK-MDR1 and Caco-2 Cell-Based Permeability Assays
- Traditional vs. Novel Drug Delivery Methods
- Unraveling the Role of hERG Channels in Drug Safety
- What factors influence drug distribution?
- How to Design and Synthesize Antibody Drug Conjugates?
- Methods of Parallel Artificial Membrane Permeability Assays
- Pharmacokinetics of Therapeutic Peptides
- What Is the Role of the Blood-Brain Barrier in Drug Delivery?
- Parameters of Pharmacokinetics: Absorption, Distribution, Metabolism, Excretion
- Key Factors Influencing Brain Distribution of Drugs
- Effects of Cytochrome P450 Metabolism on Drug Interactions
- How to Improve Drug Distribution in the Brain
- Physical and Chemical Properties of Drugs and Calculations
- What are the Pharmacokinetic Properties of the Antisense Oligonucleotides?
- What Are Compartment Models in Pharmacokinetics?
- The Rise of In Vitro Testing in Drug Development
- Predictive Modeling of Metabolic Drug Toxicity
- Overview of In Vitro Permeability Assays
-
Disease Models
- What Human Disease Models Are Available for Drug Development?
- Overview of Cardiovascular Disease Models in Drug Discovery
- Preclinical Models of Acute Liver Failure
- Animal Models of Neurodegenerative Diseases
- Summary of Advantages and Limitations of Different Oncology Animal Models
- Why Use PDX Models for Cancer Research?
- Disease Models of Diabetes Mellitus
-
Cell Biology
- Life Science Articles
- Download Center
- Trending Newsletter
Isolation Protocol of Subependymal Zone of Mice
GUIDELINE
The subependymal zone is located near the striatum in the wall of the lateral ventricle and produces olfactory bulb interneurons.
METHODS
- Mice are killed by cervical dislocation. Shears are used to cut off the head and 70% ethanol is sprayed liberally to increase sterile conditions and minimize airborne diffusion of the fur during dissection.
- Use small scissors to remove the skin and expose the skull surface. The bone is cut lengthwise along sagittal sutures without damaging the lower brain tissue.
- The skull is peeled off with curved or straight tweezers, and the brain is scooped out with a scraper and placed in a slab with cold DPBS.
- The olfactory bulb is first removed, then the midbrain is cut vertically (using the midbrain cap as a guide) to obtain a 4-5 mm thick section containing the lateral ventricles.
- Discard the rest of the tissue and separate the hemispheres one at a time.
- Tissue is opened along the corpus callosal line that separates the hippocampus, septum, and diencephalon from the cortex. This will expose the subependymal area.
- Trim the subependymal zone (SEZ) and remove all surrounding tissue. The surface of the subependymal zone is dissected carefully to obtain the thinnest tissue sections possibly. The subependymal zone is then distinguished from the parenchyma.
- Two anatomical subependymal zones of each brain are transferred into p6 holes with sterile cold DPBS and cut into 3-4 small pieces with a small scalpel.
NOTES
- Clean work surfaces thoroughly with 70% ethanol to avoid contamination. The anatomical instrument is sterilized by placing it in a glass beaker containing 70% ethanol.
- p6 well culture plastic plates are filled with cold sterile DPBS, and it is used to place and clean brains of mice before dissection. Place the plate on the ice.
- Based on the number of mice and the experience of the researchers, estimate the time required to complete the dissection. Be quick!
RELATED PRODUCTS & SERVICES
For research use only. Not for any other purpose.