MKN-45

Specification
Technical Resources
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Cat.No.

CSC-C0453

Description

Established from the poorly differentiated adenocarcinoma of the stomach (medullary type) of a 62-year-old woman

Species

Human

Source

gastric adenocarcinoma

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

monolayer

Morphology

both spindle-shaped or oval cells growing in monolayers and single round cells or clumps in suspension

Karyotype

Human hypertriploid karyotype with 8% polyploidy - 70-73<3n>XX/XXX, -X, -2, -2, -4, -5, -5, +6, -11, -13, -13, -14, -18, -19, -20, -21, -22, +20mar, add(X)(q1?), der(1)(1pter->p21::1p11->q32::1q44->q11::?), i(2p), der(3)t(3;?9)(p14;q13/21), del(4)(q21), d

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Product Sheets

Datasheet

No 'Material Safety Data Sheet' available yet

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review

How to remove fibroblasts by enzymatic digestion in the process of purifying tumor cells?

Fibroblasts are more sensitive to enzymes, so they can be removed more easily than tumor cells when digested with trypsin or collagenase. The digestion can be observed under the microscope and terminated immediately after the fibroblasts are shed, and the treatment can be repeated several times to obtain purer tumor cells.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Detailed instruction

Each product comes with a detailed instruction manual that includes the protocol for that cell culture, media composition, etc., which is very friendly for novice experimenters.

23 Apr 2022

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