HEP-3B

Specification
Recommended Products

Cat.No.

CSC-C0245

Description

Established from the tumor tissue of an 8-year-old black boy in 1976.The cells contain a 2.3 kb integrated hepatitis B virus genome fragment. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. Cells were described to produce a variety of proteins, e.g. alpha-fetoprotein, albumin, transferrin, alpha2-macroglobulin, alpha1-antitrypsin, haptoglobin and others.

Species

Homo sapiens, human

Source

Liver

Recommended Medium

EMEM + 10% h.i. FBS

Culture Properties

Monolayer

Morphology

epithelial-like cells growing as monolayers

STR DNA Profile

Amelogenin: X
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
THO1: 6,7
TPOX: 9
vWA: 17"

Karyotype

Human hypotriploid stemline with near tetraploid side line (6%) - 63 (60-64)<3n>XX, -Y, -1, -1, -2, -3, -4, -8, -9, -13, +der(11)t(1;11)(q11;q14)x2, der(?15)t(1;?15)(q11;q24), i(7p)

Applications

The HEP-3B cell line can be used as a suitable transfection host

Quality Control

Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 (+), cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, e

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x106 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1041X

CS-001Y

For research use only. Not for any other purpose.