Featured Products
- Adipose Tissue-Derived Stem Cells
- Human Neurons
- Mouse Probe
- Whole Chromosome Painting Probes
- Hepatic Cells
- Renal Cells
- In Vitro ADME Kits
- Tissue Microarray
- Tissue Blocks
- Tissue Sections
- FFPE Cell Pellet
- Probe
- Centromere Probes
- Telomere Probes
- Satellite Enumeration Probes
- Subtelomere Specific Probes
- Bacterial Probes
- ISH/FISH Probes
- Exosome Isolation Kit
- Human Adult Stem Cells
- Mouse Stem Cells
- iPSCs
- Mouse Embryonic Stem Cells
- iPSC Differentiation Kits
- Mesenchymal Stem Cells
- Immortalized Human Cells
- Immortalized Murine Cells
- Cell Immortalization Kit
- Adipose Cells
- Cardiac Cells
- Dermal Cells
- Epidermal Cells
- Peripheral Blood Mononuclear Cells
- Umbilical Cord Cells
- Monkey Primary Cells
- Mouse Primary Cells
- Breast Tumor Cells
- Colorectal Tumor Cells
- Esophageal Tumor Cells
- Lung Tumor Cells
- Leukemia/Lymphoma/Myeloma Cells
- Ovarian Tumor Cells
- Pancreatic Tumor Cells
- Mouse Tumor Cells
Our Promise to You
Guaranteed product quality, expert customer support
Combined Immunochemistry and Live Imaging of Fluorescent Protein Expressing Neurons in Mouse Brain
The Immunocytochemistry and Related Techniques, 2015: 357-373.
Combined Immunochemistry and Live Imaging of Fluorescent Protein Expressing Neurons in Mouse Brain
Authors: Empson R M, Tantirigama M L S, Oswald M J, et al.
https://link.springer.com/protocol/10.1007/978-1-4939-2313-7_19/fulltext.html
https://link.springer.com/protocol/10.1007/978-1-4939-2313-7_19/fulltext.html
Abstract
The use of transgenic mice expressing fluorescent proteins to report a specific protein or to identify specific groups of neurons in the brain is revolutionizing many different aspects of neuroscience. Here we use an example of a GFP-expressing reporter mouse from the GENSAT project that allows identification of a specific group of neurons in the mouse cortex. Live GFP detection facilitates identification of the neurons for whole-cell patch clamp electrophysiological recording to probe their functional properties. Post hoc immunohistochemistry allows specific reconstruction of the shape of the recorded neuron; this together with the detection of other co-expressed proteins helps confirm the functional identity of specific neuron types. Approaches such as these are beginning to progress the major task of untangling the complexity of a variety of brain circuits.