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Human Renal Glomerular Endothelial Cells

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Renal glomerular endothelial cells (GEC) are a specialized microvascular cell type involved in the regulation of glomerular ultrafiltration. They form the inner part of the filtration barrier and are involved in pathophysiological processes in the glomerulum. They constitutively synthesize bio-active molecules, to which this basal activity can be chronically augmented by various inflammatory and thrombotic agents. GEC injury exerts significant influences on the progression and repair process of glomerular disease. When the glomerular lesion is severe, angiogenesis is prevented due to endothelial cell injury, with subsequent sclerosis taking place in the impaired region. These glomerular endothelial cell injuries inevitably affect mesangial and epithelial cells and presumably modify the progression of renal disease by reciprocally interacting with them. Because of difficulties associated with the culture, cloning and propagation, the biological properties of these cells remain largely unknown.

HRGEC are isolated from human kidneys. HRGEC are cryopreserved after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HRGEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM), and by the formation of microtublar structure in vitro. HRGEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRGEC are guaranteed to expand beyond 15 population doublings at the conditions specified by Creative Bioarray.
Recommended Medium
It is recommended to use Endothelial Cell Medium for the culturing of HRGEC in vitro.
Storage and Shipping
ship in dry ice; store in liquid nitrogen
Cell Type
Endothelial Cells
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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