Porcine Liver Kupffer Cells are derived from porcine liver tissue. Cells are grown in tissue culture flasks and incubated in Cell Biologics’ Cell Culture Medium for 2-5 days. Prior to shipping, cells at passage 0 are detached from flasks and cryopreserved in vials. Each vial contains 3 x10^6 cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of markers using antibodies, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Porcine Liver Kupffer Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
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