Human Hypertension Vein Fibroblasts

Specification
Recommended Products

Cat.No.

CSC-C5003J

Description

Human hypertension vein fibroblasts sre isolated from the vena cava of human donor that has been diagnosed with hypertension. Human hypertension vein fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in complete growth medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml.

Species

Human

Source

Vein

Recommended Medium

SuperCult® Human Fibroblasts Growth Medium Kit

Cell Type

Fibroblast

Disease

Diseased

Quality Control

Human hypertension vein fibroblasts are negative for bacteria, yeast, fungi and mycoplasma.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1466J

SuperCult® Human Fibroblasts Growth Medium Kit

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review

How to deal with cell clumping?

It is normal for some suspension cells to grow in clusters. Most suspension cells are likely to grow in clusters when the cell density is very high, and the clustered cells will easily die and evolve into flocs, which will spill over to the surrounding suspension cells, so it is necessary to control the cell density when culturing suspension cells. If there are cell clusters, you can remove some of the larger cell clusters through the cell sieve, or you can try the following method: collect the cell suspension into a 15 ml centrifuge tube, let it stand for about 20 min, and then carefully take the upper layer of the cell supernatant for culture (this method can only remove some of the larger cell clusters).

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Convenient

Our laboratory purchases many types of cell products from Creative Bioarray and preserves them, which greatly facilitates our experiments.

19 Dec 2022

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