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Porcine Skeletla Muscle Microvascular Endothelial Cells
- Specification
- Recommended Products
Cat.No.
CSC-C8658W
Description
Porcine Skeletal Muscle Microvascular Endothelial Cells from Creative Bioarray are isolated from skeletal muscle tissue of porcine. Porcine Skeletal Muscle Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated inCreative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
Species
Porcine
Source
Skeletla Muscle
Cell Type
Endothelial
Disease
Normal
Quality Control
Porcine Skeletal Muscle Microvascular Endothelial Cells are tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Porcine Skeletal Muscle Microvascular Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray.Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What is the role of sodium pyruvate in the culture medium?
A:
Sodium pyruvate can be used as an alternative carbon source in cell culture, and although cells prefer glucose as a carbon source, they can also metabolize sodium pyruvate if glucose is not available.
Average Rating: 5.0 | 1 Scientist has reviewed this product
New discoveries
This cell product is easy to culture and brings convenience to our experiments.
18 May 2023
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