Human Brain Vascular Fibroblasts

Specification
Recommended Products

Cat.No.

CSC-8239W

Description

Human Primary Brain Vascular Fibroblasts are isolated from normal human brain tissue. Human Primary Brain Vascular Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml.

Species

Human

Source

Brain

Morphology

Fibroblasts

Applications

Human Primary Brain Vascular Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Cell Type

Fibroblast

Disease

Normal

Quality Control

Human Primary Brain Vascular Fibroblasts are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Human Primary Brain Vascular Fibroblasts are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray.Repeated freezing and thawing of cells is not recommended.

Storage and Shipping

We ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

How do I use trypsin for adherent cell passaging?

Generally use trypsin-EDTA concentration of 0.25% trypsin-0.53 mM EDTA.2Na or 0.05% trypsin-0.02 mM EDTA.2Na. When the concentration of the digestive solution is too high, it is easy to cause the increase of cell debris in the medium and the increase of black scum. It is recommended to use 0.05% trypsin for digestion in the routine cell passaging. For the hard to digested cells can be digested with 0.25% trypsin, when the cell density is too high more than 80%, step-by-step digestion method is used.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Original and ingenious

Creative Bioarray's cell products are original and ingenious.

10 Sep 2023

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