Porcine Kidney Fibroblasts

Specification
Recommended Products

Cat.No.

CSC-C4891L

Description

Porcine Kidney Fibroblasts from Creative Bioarray are isolated from kidney tissue of porcine. Porcine Kidney Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution and incubated in Creative Bioarray Complete Growth Medium. The cells are shipped at passage 2. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen.

Species

Porcine

Source

Kidney

Recommended Medium

SuperCult® Porcine Kidney Fibroblast Medium

Cell Type

Fibroblast

Disease

Normal

Quality Control

Porcine Primary Kidney Fibroblasts are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Porcine Primary Kidney Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

Why is it that many cells have difficulty attaching to the wall after resuscitation?

Ignoring the culture medium, the main consideration is that the cells are in poor condition when frozen or that the action is too slow during resuscitation leading to cell death. Remember that the most important thing is to thaw fast, and shake the cryotube from time to time so that it passes through the most vulnerable temperature band (-5 to 0°C) as quickly as possible.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

High Quality

The cell products enabled us to get accurate and reliable results from our experiments.

12 Aug 2023

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