Porcine Primary Hepatic Stellate Cells from Cell Biologics are isolated from porcine liver tissue. Cells are grown in tissue culture flasks and incubated in Cell Biologics’ Cell Culture Medium for 2-5 days. Prior to shipping, cells at passage 0 are detached from flasks and cryopreserved in vials. Each vial contains 0.5x10^6 cells per ml and is delivered frozen.
Porcine Primary Hepatic Stellate Cells from Creative Bioarray are tested for expression of markers using antibodies, Desmin or α-actin by Immunofluorescence Staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Creative Bioarray. Hepatic Stellate Cells are not recommend for expanding or long term cultures since these cells would differentiate to become fibroblat-like cells immediately after plating and they will not proliferate in culture.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.