Porcine Thyroid Epithelial Cells

Specification
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Cat.No.

CSC-C9257J

Description

Porcine Thyroid Epithelial Cells from Creative Bioarray are isolated from thyroid tissue of porcine. Porcine Thyroid Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’s Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Species

Porcine

Source

Thyroid

Recommended Medium

Complete Epithelial Cell Medium

Cell Type

Epithelial

Disease

Normal

Storage and Shipping

We ship frozen cells on dry ice. Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Never can primary cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

What should be noted in the transmission of primary cultures?

Do not rush to pass the cells until they have grown enough to cover most of the surface of the bottom wall of the flask; cells in primary culture are mostly mixed growth, and different cells have different digestion times, so pay attention to timely processing according to the need, and isolate and purify the cells needed according to the tolerance time of different cells to trypsin; The cells should be blown gently to minimize the damage to the cells; the number of cells should be inoculated more during the passaging, so that the cells can adapt to the new environment as soon as possible to facilitate cell survival and value-added; the tissue pieces shed with the digestion and separation can also be passed into the new culture bottle.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Easy to culture

We use these products and they are easy to culture and grow.

10 May 2023

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