Porcine Intestinal Mesenteric Vascular Endothelial Cells

Specification
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Cat.No.

CSC-C8637W

Description

Porcine Intestinal Mesenteric Vascular Endothelial Cells from Creative Bioarray are isolated from intestinal mesenteric vascular tissue of porcine. Porcine Intestinal Mesenteric Vascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.

Species

Porcine

Source

Mesentery

Cell Type

Endothelial

Disease

Normal

Quality Control

Porcine Intestinal Mesenteric Vascular Endothelial Cells are tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Porcine Intestinal Mesenteric Vascular Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray.Repeated freezing and thawing of cells is not recommended.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

Why use gelatin coating?

Because of the special characteristics of primary cell apposition, when the apposed primary cells are transferred to other experimental vessels after digestion (e.g. glass crawl, culture plate, confocal dish, etc.), it is necessary to coat the experimental vessels to enhance the cell apposition and avoid affecting the experiment due to the lack of cell apposition; the coating conditions are often chosen from rat tail collagen I (2-5 μg/cm2), poly-Lysine PLL (0.1 mg/ml ), gelatin (0.1%), depending on the cell type. Cells in suspension/semi-suspension do not need to be coated.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Excellent quality

We have chosen this product as the most affordable on the market.

12 May 2023

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