Porcine Bone Marrow Neutrophils

Specification
Recommended Products

Cat.No.

CSC-C4923L

Description

Porcine Bone Marrow Neutrophils are derived from porcine tibia. Each vial contains 10 x10^6 cells in suspension. Cells from Creative Bioarray are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Species

Porcine

Source

Bone Marrow

Recommended Medium

SuperCult® Complete Neutrophil Medium

Cell Type

Neutrophil

Disease

Normal

Quality Control

Porcine Bone Marrow Neutrophils are negative for bacteria, yeast, fungi, and mycoplasma.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1088X

SuperCult® Complete Neutrophil Medium

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review

The black spot has been created, how do I proceed?

If it is suspension cells: collect the cell supernatant and centrifuge it slowly (500-600 rpm/min, 5-6 min) and replace it with a new culture bottle; if it is wall-adherent cells: wash the cells with PBS for 2-3 times, when washing, gently tap the bottle to let the debris and particles that are not firmly adhered to the wall fall off, and then discard the PBS, digest the cells by adding low concentration trypsin, such as 0.05% trypsin, for 1 min or so, let particles and fragments in the cell interstices fall off, and then digest the cells normally. around, let the particles and fragments in the cell gap fall off, remove the low concentration of trypsin, then digest the cells normally, centrifuge the collected cell suspension slowly (500-600 rpm/min, 5-6 min) and replace it with a new culture flask, and you can try to increase the concentration of serum appropriately for culture.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Professional

The quality of products is top-notch and the customer service is friendly and helpful. I look forward to working with them in the future.

02 Aug 2023

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