Neutrophil Medium is a complete medium designed for the culture of murine neutrophils. It was tested and optimized with mouse or rat neutrophil culture in vitro. It is formulated for use with 5% CO2 and 95% air in a humidified incubator.
The medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with murine neutrophil culture supplement, antibiotics, and fetal bovine serum.
Storage and Shipping
Store the basal medium at 2-8 °C. Store cell growth supplement, fetal bovine serum (FBS) and antibiotics at -20 °C. The complete cell culture medium with Supplement Kit can be kept in 4 °C for two months. Protect from light.
To assure sterility, the prepared medium should be filtered with a 0.2 um filter after 2 weeks or if there is concern that sterility was compromised during the supplementation process.
Ice pack and dry ice.
Neutrophil Medium Supplement Kit includes:
5.0 ML MEM Non-Essential Amino Acids Solution (100x)
5.0 ML L-Glutamine (100x)
5.0 ML Penicillin-Streptomycin
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