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Human Bladder Epithelial Cells
- Specification
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Never can cryopreserved cells be kept at -20 °C.
- Q & A
- Customer Review
Human Bladder Epithelial Cells from Creative Bioarray are isolated from normal human bladder tissue. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials.
Our Primary bladder epithelial cells have been tested for the expression of the following biomarkers by immunocytochemistry staining: CK5 (+), CK7 (+), CK8 (+), CK18 (+), P63(nuclear) (+), Adherens junction (AJ) protein E-cadherin (+), Tight junction (TJ) protein - Zona occludens 1(ZO-1) (+) and α-smooth muscle Actin (-).
These cells proliferate very slowly and may take up to 10-14 days to fully recover from cryopreservation. After passaging, the cells may take about 7-10 days to reach 70-90% confluence, depending on the seeding density.
It is recommended to use SuperCult® Complete Human Epithelial Cell Medium (cat# CM-1098X) for the culturing of Human Bladder Epithelial Cells.
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Great products and excellent support
1) High-quality product 2) Very well organised and excellent support from product delivery and experiment design practicalities.
13 Nov 2022
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