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Rat Primary Kidney Proximal Tubular Epithelial Cells
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Cat.No.
CSC-C4162X
Description
Rat Kidney Proximal Tubular Epithelial Cells from Creative Bioarray are isolated from tissue of 6-8 week old laboratory Sprague–Dawley Rats. Rat Kidney Proximal Tubular Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^5 cells and is delivered frozen.
Species
Rat
Source
Kidney
Recommended Medium
SuperCult® Rat Epithelial Cell Medium
Morphology
Epithelial
Applications
Model for studies of normal and abnormal biology of the renal proximal tubule epithelium; Renal toxicity; Renal injury in diseases
Cell Type
Epithelial
Disease
Normal
Quality Control
Rat Kidney Proximal Tubular Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Kidney Proximal Tubular Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell interaction, adhesion, PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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