Human Dermal Epidermal Keratinocytes
Cat.No.: CSC-C9225J
Species: Human
Source: Epidermis; Skin
Cell Type: Keratinocyte
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Cat.No.
CSC-C9225J
Description
Human Dermal Epidermal Keratinocytes from Creative Bioarray are isolated from normal human skin tissue. Human Dermal Epidermal Keratinocytes are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml. The method we use to isolate epidermal keratinocytes was developed based on a combination of established and our proprietary methods. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Species
                                Human
                            Source
                                Epidermis; Skin
                            Recommended Medium
                                Human Complete Epithelial Cell Medium
                            Cell Type
                                Keratinocyte
                            Disease
                                Normal
                            Storage and Shipping
                                We ship frozen cells on dry ice. Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Never can primary cells be kept at -20 °C.
                            Citation Guidance
                        
                            If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
                            
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