Chronic Myeloid Leukemia Bone Marrow CD34+ Cells(Chronic Phase)

Specification
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Cat.No.

CSC-C4545X

Description

Chronic Myeloid Leukemia-Bone Marrow-CD34+ Cells are isolated from the bone marrow using a direct CD34 MicroBead labeling system. Chronic Myeloid Leukemia-Bone Marrow-CD34+ Cells are available in the chronic phase and the acceleration or blast crisis phase.

Species

Human

Source

Bone Marrow

Cell Type

CD34+

Disease

Diseased

Storage and Shipping

Store in liquid nitrogen and ship in dry ice.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

What is the percentage of CD34+ cells in the bone marrow mononuclear cell population?

CD34+ cells make up about 2% of the population in bone marrow mononuclear cells.

How to improve the post-thaw yield and viability of cryopreserved Human Bone Marrow CD34+ Cells?

We recommend the following thawing procedure: Rapidly thaw the cryovial in a 37°C water bath (1-2 min). Remove the vial before all the ice is thawed. Decontaminate the cryovial with 70% ethanol and move it to a biosafety cabinet and keep on ice. While on ice, transfer contents of the vial to a 15mL conical tube. Rinse the cryovial with 1mL of thawing media and slowly transfer to the cells to the conical tube. Slowly pipet 10X the vial volume of thawing media into the conical tube. Take a sample for counting and viability assessment. Centrifuge the cells at 4°C at 300 x g for 5 minutes to pellet cells. Aspirate the supernatant carefully, leaving a few mLs behind. Re-suspend cell pellet in the assay media of your choice.

What are the size of CD34+ Cells?

When resting, they are about 8-10 microns.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Active

Creative Bioarray's cell products are highly active and performed well in experiments.

14 Nov 2021

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