eNOS KO Mouse Tracheal and Bronchial Epithelial Cells from Creative Bioarray are isolated from tracheal and bronchial tissue of pathogen-free laboratory mice. eNOS KO Mouse Tracheal and Bronchial Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. The method we use to isolate epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.
eNOS KO Mouse Tracheal and Bronchial Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology). eNOS KO Mouse Tracheal and Bronchial Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
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