eNOS KO Mouse Ovarian Fibroblasts

Cat.No.: CSC-C8514W

Species: Mouse

Source: Ovary

Cell Type: Fibroblast

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Cat.No.

CSC-C8514W

Description

eNOS KO Mouse Ovarian Fibroblasts from Creative Bioarray are isolated from ovarian tissue of pathogen-free laboratory mice. eNOS KO Mouse Ovarian Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.

Species

Mouse

Source

Ovary

Cell Type

Fibroblast

Disease

Normal

Quality Control

eNOS KO Mouse Ovarian Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. eNOS KO Mouse Ovarian Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.