ActoFactor™ Human Interleukin 4 (HEK293)
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Cat.No.
CSC-CTK0343G
Description
Interleukin 4 (IL-4) is a cytokine that promotes the differentiation of T cells into Th2 cells. It is produced by activated CD4+ T cells, basophils, and mast cells, and involved in allergic responses and the regulation of immune responses.
Species
Human
Source
HEK293
GeneID
3565
Accession Number
P05112
N-terminal Sequence
Ser₈₇-Ile-Glu-Glu-Ala-Val-Pro-Ala-Val-(Cys)
Synonymous Name
IL-4, Interleukin 4, BSF-1 (B cell Stimulatory Factor 1)
Molecular Mass
Approximately 15.1 kDa (reduced), 13 and 19 kDa (non-reduced), a monomeric glycosylated polypeptide chain.
Size
10ug, 100μg, 500μg, 1000μg, Custom
Purity
>95%
Activity
Fully biologically active when compared to standard. The ED50 for IL-4, as determined by a cell proliferation assay using TF-1 human erythroleukemic cells (Kitamura, T. et al. (1989) J. Cell Physiol. 140:323), is between 0.0400 and 0.320 ng/mL, corresponding to a specific activity of > 1.0 x 10⁷ IU/mg.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 1x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA) of less than 0.2 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Usage
This GMP product can be for research use or further manufacturing use.(NOT FOR HUMAN USE)
Storage
Store lyophilized cytokine at -20°C to -80°C.
Shipping
Dry ice
Citation Guidance
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