Mouse Endothelial Cell Medium is a complete medium designed for the culture of mouse endothelial cells. It was tested and optimized with mouse endothelial cell growth and proliferation in vitro. It is formulated for use with 5% CO2 and 95% air in a humidified incubator. We highly suggest using the media as the specific medium helps to maintain cell growth in the first 2 weeks.
The medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with endothelial cell growth supplement, antibiotics, and fetal bovine serum.
Storage and Shipping
Store the basal medium at 2-8 °C. Store endothelial cell growth supplement, fetal bovine serum (FBS) and antibiotics at -20 °C. The complete cell culture medium with Supplement Kit can be kept in 4 °C for two months. Protect from light.
To assure sterility, the prepared medium should be filtered with a 0.2 um filter after 2 weeks or if there is concern that sterility was compromised during the supplementation process.
Ice pack and dry ice.
Endothelial Cell Medium Supplement Kit includes:
0.5 ML VEGF
0.5 ML EGF
1.0 ML ECGS
0.5 ML Heparin
0.5 ML Hydrocortisone
5.0 ML L-Glutamine
5.0 ML Antibiotic-Antimycotic Solution
25.0 ML FBS
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.