Normal PB CD4+/CD25+ Regulatory T Cells

Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Besides, dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. During wound healing, dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype.HLF from Bioarray Research Laboratories are isolated from human lymph nodes. HLF are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HLF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HLF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLF are guaranteed to further expand for 15 population doublings at the condition provided by Bioarray Research Laboratories.

Description: Creative Bioarray's Human T Cells-Negatively Selected (HTC) are purified from healthy donors and ready to use in studies of T cell biology. HTC can respond to mitogens or anti-CD3 antibodies with robust proliferation and secretion of interferonγ, tumor necrosis factor α and interleukin-6.

Creative Bioarray's HTC are cryopreserved immediately after isolation and purification to ensure preservation of all circulating T cell subpopulations and antigen specificities. Our HTC are quality tested via flow cytometry to ensure depletion on undesired cell types and are typically ≥80% CD3 positive. Creative Bioarray's HTC are positive for CD3 and contain both CD4 and CD8 subsets. Creative Bioarray's HTC are negative for other lineage markers such as CD14, CD19 and CD56

Description: Creative Bioarray's Human B Cells (HBC) provide a source of human antibody producing cells. HBC contain B cells from the circulation with a variety of antibody specificities. HBC are used for antibody production, studies of antigen presentation function and many other facets of immunology.

Creative Bioarray's HBC are cryopreserved immediately following purification from HPBMC. Our HBC are quality tested via flow cytometry to ensure proper expression of CD19 and depletion of undesired cell types. HBC express CD19 but lack CD3, CD14 or CD56.

Cell Features:
HBC are cryopreserved upon isolation and purification.
HBC are extensively tested for quality and optimal performance.
Creative Bioarray guarantees performance and quality.

Description: NPB-Pan T Cells are negatively isolated from mononuclear cells using an indirect immunomagnetic Pan-T labeling system.

Description: First, NPB-CD8+ T Cells (CTL) are negatively isolated from Mononuclear Cells using immunomagnetic CD8+ T cell isolation kit. Next, CD45RO MicroBeads are used to deplete the CD45RO+ cell population, leaving a purified CD8+/CD45RA+ Naïve T Cell population.

Description: NPB-CD8+ Cytotoxic T cells are negatively isolated from Mononuclear Cells using an indirect immunomagnetic CD8+ T cell labeling system.