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Mouse Tumor-Associated Endothelial Cells-4T1

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Mouse Tumor-Associated Endothelial Cells (Mouse Breast Cancer Origin, MTMMECM) from Creative Bioarray were isolated from pathogen-free laboratory nude mice. MTMMECM from Creative Bioarray were isolated from human xenograft of pathogen-free laboratory nude mice. Mouse breast adenocarcinoma epithelial cells (4T1) were subcutaneously injected into the nude mice for 4-6 weeks and the tumor tissues were then harvested for cell culture. MTMMECM were cultured in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 14-35 days. Cells were detached from flasks and immediately cryo-preserved in vials. Cells will be delivered in a frozen vial and each vial contains 0.5x10^6 cells/ml. The method we use to isolate tumor-associated endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
Quality Control
MTMMECM are tested for expression of markers using antibody, Ve-Cadherin (CD144, VE-Cadherin Antibody, C-19, sc6458 Santa Cruz); or CD31/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. MTMMECM are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.
Cell Type
Endothelial Cells
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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Tel: 1-631-626-9181
Fax: 1-631-614-7828

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