Mouse Perivascular Resident Macrophate-type Melanocytes (PVM/Ms) are isolated from normal mouse tissue and purified by flow cytometry based on F4/80 sorting. Sorted cells were validated with anti-F4/80 and anti-GSTα4 in immunohistochemical staining. The perivascular resident macrophate-type melanocytes (PVM/Ms), along with cochlear microvascular endothelial cells and cochlear pericytes, are important for maintaining the inner ear homeostasis. The PVM/Ms have been found to be essential in regulating the permeability of the intrastrial fluid-blood barrier. The cells can be used in vascular dysfunction-related hearing loss study.
Melanocyte-like, have unique dendritic processes
The cells are negative for mycoplasma, bacteria, yeast and fungi.
Storage and Shipping
ship in dry ice; store in liquid nitrogen
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