Mouse Adenocarcinoma Epithelial Cells (Human Breast Cancer Origin, MTMMEPIC.H) from Creative Bioarray were isolated from human xenograft of pathogen-free laboratory nude mice. Human breast adenocarcinoma epithelial cells (MDA-MB-231) were subcutaneously injected into the nude mice for 4-6 weeks and the tumor tissues were then harvested for cell culture. MTMMEPIC.H were cultured in a T75 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’s Culture Complete Growth Medium generally for 14-35 days. Cells were detached from flasks and immediately cryo-preserved in vials. Cells will be delivered in a frozen vial and each vial contains 0.5x10^6 cells/ml. Cells can be expanded for 8-12 passages at a split ratio of 1:3 under the cell culture conditions specified by Creative Bioarray.
Complete Epithelial Cell Medium
Storage and Shipping
We ship frozen cells on dry ice. Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Never can primary cells be kept at -20 °C.
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