ActoFactor™ Recombinant Mouse Interleukin 9

CSC-CTK0687

Interleukin (IL)-9 is known to regulate many cell types involved in T-helper type 2 responses classically associated with asthma, including B- and T-lymphocytes, mast cells, eosinophils and epithelial cells. Growing evidence obtained from human genomic analysis and antigen-challenged transgenic mice suggests that interleukin-9 (IL-9) is a candidate factor in immunoglobulin E (IgE) production and thus is thought to be associated with bronchial inflammation and bronchial hyperresponsiveness (BHR). Recombinant mouse IL-9 produced in E. coli is a single, non- glycosylated polypeptide chain of 127amino acids and a molecular mass of 14,166 Dalton.

Mouse

Mouse Il9 expressed in E.coli

14.2 kDa

CAT# CSC-CTK0687-10 (10 μg); CAT# CSC-CTK0687-50 (50 μg)

E.coli

Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.

Recombinant murine IL-9 is fully biologically active when compared to a standard. The ED50, as determined by the dose-dependant stimulation of human MO7e cells, is < 0.5 ng/ml corresponding to a specific activity of 2 x 10^6 IU/mg.

Less than 1 EU/μg.

Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) solution containing no additives.

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu IL-9 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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