ActoFactor™ Recombinant Mouse Betacellulin

CSC-CTK0674

Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant murine Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Mouse

Mouse Btc expressed in E.coli

32 kDa

CAT# CSC-CTK0674-20 (20 μg); CAT# CSC-CTK0674-100 (100 μg)

E.coli

Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.

Determined by its ability to stimulate the proliferation of mouse Balb/3T3 cells. The expected ED50 is 1 x 10^8 units/mg.

Less than 1 EU/μg.

Lyophilized from a sterile-filtered protein solution with no additives.

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

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