ActoFactor™ Recombinant Human Wingless-type MMTV integration site family, member 7A

CSC-CTK0618

Wnt-7a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. It is expressed in placenta, kidney, testis, uterus, fetal lung and fetal and adult brain. Most Wnt proteins can signal though a mechanism called the canonical Wnt pathway, in which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of β-Catenein degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors and induces the expression of numerous genes. Increased Wnt/β-Catenein signaling is associated with tumorigenesis in a diverse set of human cancers. However, Wnt-7a/Frizzled-9 signaling has been shown to act as a tumor suppressor in non-small cell lung cancers. Recombinant human Wnt-7a is a 35.5 kDa glycoprotein containing 318 amino acids. Due to glycosylation, Wnt-7a migrates between 40-55 kDa by SDS-PAGE gel under unreduced conditions.

Human

Human WNT7A expressed in HEK293 cells

35.5 kDa

CAT# CSC-CTK0618-15 (15 μg); CAT# CSC-CTK0618-75 (75 μg)

HEK293 cells

Greater than 80% as determined by SDS-PAGE and RP-HPLC analysis.

Determined by its ability to inhibit Wnt3a induced alkaline phosphatase production in MC3T3-E1 cells. The expected ED50 for this effect is 40-60 ng/ml.

Less than 1 EU/μg.

Sterile-filtered through a 0.2 micron filter and ly ophilized from 10 mM sodium phosphate (pH 7.5), 500 mM NaCl, 0.5% CHAPS.

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of ≤0.2 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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