ActoFactor™ Recombinant Human WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2

CSC-CTK0638

Growth and differentiation factor-associated serum protein-1 (GASP-1) is a secreted inhibitory TGF-β binding protein that contains multiple protease inhibitor structural domains. It is expressed primarily in the ovary, testis, and brain, and can act as a potent soluble inhibitor of myostatin and GDF-11, but not Activin-A. The GASP-1 gene encodes a 571 amino acid protein that contains a 29 amino acid secretion signal sequence, and multiple identifiable structural features, including a WAP domain, a follistatin/Kazal domain, an immunoglobulin domain, two tandem Kunitz domains, and a netrin domain. Recombinant human GASP-1 is a 542 amino acid protein that migrates at an apparent molecular weight of approximately 55-66 kDa by SDS-PAGE analysis under non-reducing conditions.

Human

Human WFIKKN2 expressed in CHO cells

N/A

CAT# CSC-CTK0638-25 (25 μg); CAT# CSC-CTK0638-125 (125 μg)

CHO cells

Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.

Determined by its ability to inhibit human Myostatin (GDF-8) activity in MCP-11 cells. The ED50 for this effect is 0.0025-0.0040 μg/ml in the presence of 5ng/ml of human Myostatin (GDF-8).

Less than 1 EU/μg.

Sterile-filtered through a 0.2 micron filter and ly ophilized from 10 mM sodium phosphate (pH 7.8).

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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