ActoFactor™ Recombinant Human Transforming growth factor, beta 3

CSC-CTK0330

Transforming growth factor-betas (TGF-betas) are multifunctional peptides that regulate growth and differentiation in a variety of cells. The transforming growth factor-beta (TGF-beta) family of growth modulators play critical roles in tissue development and maintenance. Recent data suggest that individual TGF-beta isoforms (TGF-beta 1, -beta 2 and -beta 3) have overlapping yet distinct biological actions and target cell specificities, both in developing and adult tissues. Transforming Growth Factor-beta 3 (TGF-beta 3) is a new isoform of the TGF-beta superfamily and is presumed to play an important role in wound repair and scarring. TGF-beta 3 is believed to be involved in osteoblast proliferation, chemotaxis, and collagen synthesis. Recombinant human TGF-beta 3 produced by transient expression of TGF-β3 in non-transgenic plants contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC). This product does not contain any animal-derived components or impurities. Recombinant human TGF-β3 is a 27.2 kDa protein composed of two identical 118 amino acid polypeptide chains linked by a single disulfide bond.

Human

Human TGFB3 expressed in Nicotiana benthamiana

27.2 kDa

CAT# CSC-CTK0330-5 (5 μg); CAT# CSC-CTK0330-25 (25 μg)

Nicotiana benthamiana

Greater than 97% as determined by SDS-PAGE analysis.

The biological activity of TGF-β3 was measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation. The ED50 was found to be ≤50 ng/ml.

Less than 0.04 EU/μg.

Lyophilized from a 0.05 M Tris-HCl buffer (pH 7.4).

Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in water to a concentration of 5-25 ng/μl. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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