ActoFactor™ Recombinant Human Transforming growth factor, beta 2 (Insect)

CSC-CTK0327

The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β2 has been shown to exert suppressive effects on IL-2 dependent T-cell growth, and may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development. Recombinant human TGF-β2 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

Human

Human TGFB2 expressed in High-5 Insect Cells

25.0 kDa

CAT# CSC-CTK0327-5 (5 μg); CAT# CSC-CTK0327-25 (25 μg)

High-5 Insect Cells

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The ED50 was found to be ≤ 0.2 ng/ml, corresponding to a specific activity of Greater than 5 x 10^6 units/mg.

Less than 1 EU/μg.

Lyophilized from a sterile-filtered solution.

Please centrifuge product before opening the vial. The lyophilized rHu TGF-beta2 should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use

The lyophilized powder, though stable at room temperature for up to 3 months, is best stored desiccated at -20°C. Reconstituted rHu TGF-beta2 should be used immediately or stored long- term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

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