ActoFactor™ Recombinant Human Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A

CSC-CTK0640

Semaphorins are a large group of structurally related secreted, GPI-anchored and transmembrane cell signaling molecules. There are 8 major classifications (1-7) of Semaphorins characterized by the existence of a conserved 500 amino acid SEMA domain at the amino terminus. Classes 3,4,6, and 7 are found in vertebrates only, whilst class 5 is found in both vertebrates and invertebrates. Each class is then divided into additional subgroups based on shared structural characteristics. Semaphorins primarily function as axon growth cone guidance factors during neuronal development. Semaphorin 3A acts as a chemo-repellent to axons, and an inhibitor of the growth of axons by signaling through receptors, Neuropilin-1and Plexin-A. Recombinant human Semaphorin 3A is a 751 amino acid protein containing the SEMA domain, an immunoglobulin c2-like domain and a basic domain (Arg/Lys rich) near the C-terminus.

Human

Human SEMA3A expressed in CHO cells

N/A

CAT# CSC-CTK0640-25 (25 μg); CAT# CSC-CTK0640-125 (125 μg)

CHO cells

Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.

N/A

Less than 1 EU/μg.

Sterile-filtered through a 0.2 micron filter and lyophilized from 20 mM Tris (pH 8.0).

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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