ActoFactor™ Recombinant Human Platelet/endothelial cell adhesion molecule 1

CSC-CTK0305

CD31 (PECAM-1) is a transmembrane glycoprotein expressed on endothelial cells and in platelets. It is known to be involved in cell signaling and cell adhesion. PECAM that belongs to the Ig-related superfamily of adhesion molecules. It is highly expressed at endothelial cell junctions, and also expressed in platelets and in most leukocyte sub-types. The primary function of PECAM-1 is the mediation of leukocyte-endothelial cell adhesion and signal transduction. PECAM-1 has been implicated in the pathogenesis of various inflammation related disorders, including thrombosis, multiple sclerosis (MS), and rheumatoid arthritis. The human PECAM-1 gene codes for a 738 amino acid transmembrane glycoprotein containing a 118 amino acid cytoplasmic domain, a 19 amino acid transmembrane domain, and a 574 amino acid extracellular domain. Recombinant human PECAM-1 is a 572 amino acid glycoprotein comprising the extracellular domain of PECAM-1. Monomeric glycosylated PECAM-1 migrates at an apparent molecular weight of approximately 80.0-95.0 kDa by SDS-PAGE analysis under reducing conditions.

Human

Human PECAM1 expressed in HEK293 cells

N/A

CAT# CSC-CTK0305-50 (50 μg); CAT# CSC-CTK0305-250 (250 μg)

HEK293 cells

Greater than 97% as determined by SDS-PAGE and HPLC analyses.

Determined by its ability to support the adhesion of activated Jurkat cells. The expected ED50 for this effect is 1.0 -1.5 μg/ml.

Less than 1 EU/μg.

Sterile filtered through a 0.2 micron filter and ly ophilized from 10 mM sodium phosphate buffer (pH 7.5).

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

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