ActoFactor™ Recombinant Human Platelet derived growth factor AA (E. coli)

CSC-CTK0280

Recombinant Human Platelet Derived Growth Factor (rHu PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AA is a 28.5 kDa A chain homodimer protein containing 250 amino acid residues.

Human

Human PDGFA expressed in E.coli

28.5 kDa

CAT# CSC-CTK0280-10 (10 μg); CAT# CSC-CTK0280-50 (50 μg)

E.coli

Greater than 97% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.

rHu PDGF-AA is fully biologically active when compared to a standard. The ED50, as determined by the dose-dependent stimulation of the proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake), is 1.0 ng/ml corresponding to a specific activity of 10^6 U/mg.

Less than 1 EU/μg.

Sterile-filtered protein solution was lyophilized with no additives.

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted PDGF-AA should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze / thaw cycles.

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