ActoFactor™ Recombinant Human Neuropilin 1

CSC-CTK0399

VEGF (Vascular endothelial growth factor) is the only growth factor that stimulates vascular permeability. It promotes endothelial proliferation and survival, angiogenesis, vasculognesis and inhibits apoptosis. VEGF mitogenic activity is specific for endothelial cell and that makes it distinct among other growth factors. VEGF is thought to be important in the pathophysiology of neuronal and other tumors, by functioning as a promter of angiogenesis for human gliomas. Human VEGF occurs in several molecular variants and the 165 form is the most common form in most tissues. Biological activities of VEGF are not species-specific and glycosylation is not required for biological activity. Recombinant human VEGF-165 produced in plant contains 165 amino acids and a 16 a.a. histidine-based tag for a total length of 181 a.a., and has a predicted molecular mass of 21.3 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 26-28 kDa in SDS-PAGE.

Human

Human NRP1 expressed in Endosperm tissue of barley grain (Hordeum vulgare)

21.3 kDa

CAT# CSC-CTK0399-10 (10 μg); CAT# CSC-CTK0399-50 (50 μg)

Endosperm tissue of barley grain (Hordeum vulgare)

Greater than 95% as determined by SDS-PAGE analysis.

The ED50 is determined by a cell proliferation assay measuring the dose-dependent effects of serial dilutions of recombinant human VEGF-165 on Human Umbilical Vein Endothelial Cells (HUVECs) and is comparable to VEGF-165 from other commercial sources. The ED50 for this effect was found is typically below 10 ng/ml corresponding to a specific activity of >10^5 units/mg. The optimal concentration should be determined for specific applications and cell lines.

Less than 0.05 EU/μg.

Sterile filtered through a 0.2 μm filter. Lyophilized from PBS (pH 7.2).

Please centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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