ActoFactor™ Recombinant Human Neuritin 1

CSC-CTK0563

Neuritin is a neurotrophic factor, which is expressed in response to induction of neuronal activity by NGF, BDNF, NT3, and other neural stimulators. It is expressed primarily in postmitotic- differentiating neurons of the developing nervous system and in neuronal structures related to synaptic plasticity in the adult nervous system. Neuritin acts as a molecular mediator of neurite outgrowth, neuronal survival, and synaptic maturation. Recombinant human Neuritin is a covalently disulfide-linked homodimer, consisting of two 9.7 kDa polypeptide monomers, each containing 88 amino acid residues.

Human

Human NRN1 expressed in E.coli

9.7 kDa

CAT# CSC-CTK0563-20 (20 μg); CAT# CSC-CTK0563-100 (100 μg)

E.coli

Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.

Determined by a cell proliferation using rat C6 cells. The ED50 for this effect is 20.0-30.0 ng/ml.

Less than 1 EU/μg.

Sterile-filtered through a 0.2 micron filter. Lyophilized from a protein solution with no additives.

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultrapure water or aqueous buffers to a concentration of 0.1- 1.0 mg mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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