ActoFactor™ Recombinant Human KIT ligand-alpha

CSC-CTK0293

Stem Cell Growth Factor (SCGF) is a newly discovered hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. Human SCGF is a non-glycosylated 33.9 kDa species-specific cytokine of 305 amino acids that can support growth of primitive hematopoietic cells, and in combination of EPO or GM-CSF, it promotes proliferation of erthroid and myeloid progenitors, respectively.

Human

Human KITLG expressed in E.coli

33.9 kDa

CAT# CSC-CTK0293-10 (10 μg); CAT# CSC-CTK0293-50 (50 μg)

E.coli

Greater than 98% as determined by SDS-PAGE and HPLC analyses. Endotoxin level is less than

Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect is 2.5 ng/ml.

Less than 1 EU/μg.

Lyophilized from a sterile-filtered protein solution with no additives.

Please centrifuge product briefly before opening the vial. The lyophilized SCGF is soluble in water and most aqueous buffers. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1 mg/ml. It is recommended that further dilutions be made into buffer containing carrier protein (HSA or BSA) or medium containing serum.

The lyophilized SCGF, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted SCGF should be used immediately or stored long-term in undiluted working aliquots at -20°C.

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