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ActoFactor™ Recombinant Human Interleukin 10
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Cat.No.
CSC-CTK0082
Description
Human Interleukin 10 (IL-10) is a species cross-reactive cytokine produced by CD4+ as well as by some CD8+ T cells. In addition, human B cells, EBV-transformed lymphoblastoid cell lines and monocytes can produce IL-10 upon activation. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or
immunosuppressive effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation , Ig secretion and class II MHC expression. IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development. As an immunosuppressant, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by downregulating class II MHC expression. It inhibits IFN-g synthesis and Th1 differentiation in the mouse system. It can suppress the production of numerous cytokines including TNF-a, IL-1 and IL-6, as well as the synthesis of superoxide anion, reactive oxygen intermediates (ROI), and reactive nitrogen intermediates (RNI) by activated monocytes/macrophages. Recombinant human IL-10 containing 160 amino acid residues has a predicted molecular mass of approximately 18.6 kDa.
Species
Human
Product Overview
Human IL10 expressed in Sf21
Sequence
A DNA sequence encoding the mature human IL-10 protein was expressed in Sf21 insect cells.
Size
CAT# CSC-CTK0082-5 (5 μg); CAT# CSC-CTK0082-25 (25 μg)
Expression System
Sf21
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
Activity was determined in a cell proliferation assay using the murine mast cell line, MC/9 and the ED50 using this assay was typically 0.5 – 1.0 ng/ml.
Endotoxin Level
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/ml of the cytokine.
Storage & Stability
The lyophilized sample is stable for greater than six months at -20°C to -70°C. Reconstituted IL-10 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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