ActoFactor™ Recombinant Human Glucagon-Like Peptide 1

CSC-CTK0636

GLP-1 is a proglucagon-derived peptide hormone secreted primarily by intestinal L cells during feeding. Its major physiological function is stimulation of pancreatic β-cells to release appropriate amounts of insulin after glucose absorption. Other biological actions exhibited by GLP-1 include suppression of plasma glucagons levels, inhibition of gastric motility, and promotion of satiety. The secretion of GLP-1 from intestinal L cells is stimulated by nutrients, hormones, and neural inputs. On the other hand, insulin has been reported to inhibit GLP-1 release, indicating that a feedback loop mechanism regulates GLP-1 secretion. In addition to being the precursor of GLP- 1, proglucagon, whose primary structure is highly conserved in mammalian species, is also the precursor for other members of the glucagon family of peptide hormones including glicentin- related pancreatic peptide (GRPP), glucagons, and GLP-2. Recombinant human GLP-1 is a 3.3 kDa consisting of 31 amino acid residues.

Human

Human GCG expressed in E.coli

3.3 kDa

CAT# CSC-CTK0636-1 (1 mg); CAT# CSC-CTK0636-5 (5 mg)

E.coli

Greater than 95% as determined by SDS-PAGE and HPLC analysis.

N/A

Less than 1 EU/μg.

Sterile filtered through a 0.2 micron filter and lyophilized with no additives.

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu GLP-1 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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