ActoFactor™ Recombinant Human Fms-related tyrosine kinase 3 (HEK)

CSC-CTK0520

Recombinant human flt-3 Ligand stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells. Flt-3 ligand also has a tyrosine-protein kinase activity and synergizes with other CSFs and interleukins to induce growth and differentiation. Recombinant human flt-3 Ligand produced in HEK293 cells is a glycosylated polypeptide chain and migrates as a diffuse band on SDS-PAGE due to heterogeneous glycosylation at 30 kDa.

Human

Human FLT3 expressed in HEK293 cells

17.6 kDa

CAT# CSC-CTK0520-10 (10 μg); CAT# CSC-CTK0520-50 (50 μg)

HEK293 cells

Greater than 95% as determined by SDS-PAGE and HPLC analysis.

The ED50 as determined by the dose dependent proliferation of human OCI-AML5 cells is 0.5 to 1.0 ng/ml, corresponding to a specific activity of Greater than 1 x 10^6 units/mg.

Less than 1 EU/μg.

Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a protein solution with no additives.

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20 C° for future use.

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.