ActoFactor™ Recombinant Human Collagen, type XVIII, alpha 1

CSC-CTK0614

Endostatin is a naturally occurring 20 kDa polypeptide derived from the C-terminal portion of type XVIII collagen. It functions as an anti-angiogenic cytokine that is expressed in various organs with the highest levels in liver, lung and kidney. Endostatin inhibits angiogenesis by blocking the pro-angiogenic activities of VEGF and FGF-basic. Recombinant human Endostatin produced in Pichia Pastoris is a single, glycosylated polypeptide consisting of 184 amino acid residues and having a total molecular mass of 20,000 Dalton. is a 20.2 kDa protein. The Endostatin was purified using proprietary chromatographic techniques.

Human

Human COL18A1 expressed in Pichia pastoris

20.2 kDa

CAT# CSC-CTK0614-100 (100 μg); CAT# CSC-CTK0614-500 (500 μg)

Pichia pastoris

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

The activity calculated by ECE migration inhibition was found to be 50,000IU/mg.

Less than 1 EU/μg.

Sterile filtered liquid formulation 5 mg/ml in PBS.

N/A

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

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