ActoFactor™ Recombinant Human Chemokine (C-X-C motif) ligand 2

CSC-CTK0445

Three human MGSA/GRO genes encode 3 highly related chemokines, MGSA/GRO alpha, -beta and -gamma. All 3 MGSA/GRO proteins bind to the same receptors, but with differing affinities, and stimulate a number of biological responses including chemotaxis, angiogenesis and growth regulation. Recombinant human GRO-beta produced in E. coli is a single, non-glycosylated polypeptide chain of 73 amino acids and a molecular mass of 7,908 Dalton.

Human

Human CXCL2 expressed in E.coli

7.9 kDa

CAT# CSC-CTK0445-10 (10 μg); CAT# CSC-CTK0445-50 (50 μg)

E.coli

Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.

Recombinant GRO-beta is fully biologically active when compared to a standard. The biological activity is calculated by its ability to chemoattract CXCR2 transfected 293 cells using a concentration of 10-100 ng/ml.

Less than 1 EU/μg.

Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml), sterile solution containing no additives.

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu GRO-beta should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.