ActoFactor™ Recombinant Human Chemokine (C-X-C motif) ligand 12 (beta)
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Cat.No.
CSC-CTK0535
Description
Recombinant Human Stromal Cell-Derived Factor-1beta (rHu SDF-1 beta) is a recently discovered protein belonging to the a-chemokine (C-X-C) family of cytokines. Human SDF-1 beta is an 8.5 kDa protein containing 72 amino acid residues.
Species
Human
Product Overview
Human CXCL12 expressed in E.coli
Molecular Mass
8.5 kDa
Size
CAT# CSC-CTK0535-10 (10 μg); CAT# CSC-CTK0535-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Activity
The activity of rHu SDF-1 beta was determined by its ability to chemoattract human peripheral T- cells activated with PHA and IL-2. This effect was reached using a concentration range of 20.0- 80.0 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a concentrated (1 mg/ml), sterile protein solution with no additives.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized SDF-1 beta, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted human SDF-1 beta should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
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