ActoFactor™ Recombinant Human Bone morphogenetic protein 2 (E. coli)

CSC-CTK0524

Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homo-dimeric protein with an apparent molecular weight of 26 kDa. BMP-2 regulates similarly to its nearest homologue BMP-4, diverse fundamental processes during embryonic development. BMP-2 and other BMP proteins have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions.

Human

Human BMP2 expressed in E.coli

26 kDa

CAT# CSC-CTK0524-10 (10 μg); CAT# CSC-CTK0524-50 (50 μg)

E.coli

Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.

The ED50, as determined by ist ability to induce alkaline phosphatase production by ATDC-5 cells, is 0.5-1.0 μg/ml.

Less than 1 EU/μg.

BMP-2 was lyophilized from a concentrated (1 mg/ml), sterile solution containing 10 mM sodium citrate (pH 3.5).

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile 20 mM acetic acid to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu BMP-2 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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