Human Interleukin-4 for cell culture (rHu IL-4 CC) is a 15 kDa protein containing 130 amino acid residues. rHu IL-4 CC is a potent lymphoid cell growth factor, which stimulates the growth and survivability of certain B- and T-cells.
Human IL4 expressed in E.coli
CAT# CSC-CTK0222-5 (5 μg); CAT# CSC-CTK0222-25 (25 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
The biological activity was determined by a standard cell proliferation assay using a human factor-dependent cell line TF-1. The ED50 for this effect is 0.1-0.2 ng/ml, corresponding to a specific activity of Greater than 5 x 10^6 units/mg (WHO standard 88/656, NIBSC).
Lyophilized from DMEM/F12 (stabilizer: HSA)
Please centrifuge product briefly before opening the vial. The lyophilized rHu IL-4 CC should be reconstituted in 2 ml of sterile, distilled water. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-4 CC should be used immediately or divided into aliquots and stored undiluted at -20°C for future use. Please avoid repeated freeze / thaw cycles.
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