IL-24 is a member of the IL-10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. IL-10B encoded, it can induce apoptosis selectively in various cancer cells. Its overexpression of leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen- activated protein kinase 14 (MAPK7/P38) and heat shock 27 kDa protein 1 (HSPB2/HSP27) are induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have also been reported. Glycosylation is essential for activity of IL-24 that has diverse activities. At low concentrations, it induces type I proinflammatory cytokines such as IFN-γ, IL-1β, IL-12 and TNF-α. At high concentration, it is a strong inducer of apoptosis in tumor cells, but not normal cells. mda-7/IL-24 is being hailed as a 'magic bullet' for cancer gene therapy. Recombinant human Interleukin-24 produced in CHO cells is an 18.9 kDa glycoprotein, containing 161 amino acid residues, including a C-terminal His- tag.
Human IL24 expressed in CHO cells
CAT# CSC-CTK0272-20 (20 μg); CAT# CSC-CTK0272-100 (100 μg)
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
Less than 1 EU/μg.
Sterile-filtered through a 0.2 micron filter and lyophilized from 10 mM sodium phosphate buffer (pH 7.5).
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-24 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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