Recombinant human IL-22 produced in E. coli is a non-disulfide-linked homodimeric, non- glycosylated protein containing 2 x 146 amino acid chains and having a total molecular mass of 33,607 Dalton.
Human IL22 expressed in E.coli
CAT# CSC-CTK0269-10 (10 μg); CAT# CSC-CTK0269-50 (50 μg)
Greater than 97% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Recombinant human Interleukin-22 is fully biologically active when compared to a standard. The activity is determined by its ability to activate STAT following receptor-ligand interaction.
Less than 1 EU/μg.
Lyophilized from sterile-filtered protein solution.Each mg contains 1.6 μg mannitol and 1.2 μg HSA.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-22 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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