BMP-4 (Bone Morphogenetic Protein 4) is a member of the transforming growth factor β (TGF-β) superfamily. BMPs have been shown to be key regulators of embryogenesis and are known to play a role in the growth and differentiation of various cell types, including embryonic stem cells (ESCs), mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. The mature recombinant human BMP-4 is a disulfide-linked homodimeric protein consisting of two 116
amino acid residue subunits, and is generated by the proteolytic removal of the signal peptide and propeptides. The monomer has a calculated molecular mass of approximately 13 kDa, but migrates as an approximately 22 kDa protein under reducing conditions in SDS-PAGE due to glycosylation.
Human BMP4 expressed in NS0 cells
The DNA sequence encoding the human BMP-2 signal peptide and propeptide (amino acid residues 1 - 282 of human BMP-2) fused to the human BMP-4 mature chain (amino acid residues 293 - 408 of human BMP-4) (Wozney, J. et al., 1988, Science 242: 1528 - 1534) w
CAT# CSC-CTK0039-10 (10 μg); CAT# CSC-CTK0039-50 (50 μg)
Greater than 95% as determined by SDS-PAGE and visualized by silver stain
The ED50 as measured by its ability to induce alkaline phosphatase production by mouse ATDC5 chondrogenic cells (Nakamura, K. et al., 1999, Exp. Cell Res. 250: 351) is typically 10 - 30 ng/mL.
Endotoxin level is less than 1.0 EU per 1 μg of the cytokine, as determined by the LAL method.
Lyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA containing 50 μg of bovine serum albumin per 1 μg of cytokine.
It is recommended that sterile 4 mM HCl containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 μg/mL.
Storage & Stability
The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted BMP-4 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
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